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ALLPATHS-LG

ALLPATHS-LG

Description

ALLPATHS‐LG is a whole‐genome shotgun assembler that can generate high‐quality genome assemblies  using short reads. It has been designed to use reads produced by new sequencing  technology machines such as the Illumina Genome Analyzer. The current version has been  optimized for, but not necessarily limited to, reads of length 100 bases.  ALLPATHS is not designed to assemble Sanger or 454 FLX reads, or a mix of these with short reads.

ALLPATHS‐LG requires high sequence coverage of the genome in order to compensate for the shortness  of the reads. The precise coverage required depends on the length and quality of the paired reads, but  typically is of the order 100x or above. This is raw read coverage, before any error correction or filtering.

ALLPATHS‐LG requires a minimum of 2 paired‐end libraries – one short and one long. The short library  average separation size must be slightly less than twice the read size, such that the reads from a pair will  likely overlap – for example, for 100 base reads the insert size should be 180 bases. The distribution of  sizes should be as small as possible, with a standard deviation of less than 20%. The long library insert  size should be approximately 3000 bases long and can have a larger size distribution. Additional optional longer insert libraries can be used to help disambiguate larger repeat structures and may be generated at lower coverage.


Available

Version on CSC's Servers

Taito: 51503 (default), 52488


Usage

To initialize the program you need to give command:

module load biokit
After this you can start using ALLPATHS-LG commands.


Discipline

Biosciences

References


Support

servicedesk@csc.fi


Manual

More information about ALLPATHS-LG can be found from: